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Fisher Scientific maxima reverse transcriptase
Maxima Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxima+reverse+transcriptase/m+mlv+reverse+transcriptase/pmc12549399-327-10-13
Average 86 stars, based on 1 article reviews
maxima reverse transcriptase - by Bioz Stars, 2026-10
86/100 stars

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Synthesized:

Article Title: Unravelling the synergies: effects of hydrogel mechanics and biofunctionalization on mesenchymal stem cell osteogenic differentiation
Article Snippet: .. RNA samples were extracted and processed with the RNeasy micro kit (Qiagen). cDNA was synthesized from 0.1 mg of total RNA using Maxima Reverse Transcriptase (Fisher Scientific) and primed with oligo-dT primers (Fisher Scientific) and random primers (Fisher Scientific). .. QPCR was performed using a LightCycler 480 Real-Time PCR System (Roche, Meylan, France).

Article Title: Repression of apelin Furin cleavage sites provides antimetastatic strategy in colorectal cancer.
Article Snippet: Ribonucleic acid (RNA) extraction, real-time PCR and droplet digital PCR (ddPCR) For real-time PCR, total RNA was extracted using an RNA isolation kit (Macherey-Nagel) including DNase treatment (Qiagen), according to the manufacturer’s instructions, and qPCR data were acquired with the StepOnePlusTM Real-Time PCR System (Applied Biosystems, Courtaboeuf, France), as previously described (Scamuffa et al, 2008). .. For ddPCR, cDNA was synthesized from 2 μg of total RNA using Maxima Reverse Transcriptase (Fisher Scientific) and primed with oligo-dT primers (Fisher Scientific) and random primers (Fisher Scientific). .. PCR were performed in a final volume of 22 μl containing the required QX200 ddPCR EvaGreen Supermix (Bio-Rad) with a final concentration of 150 nM of each primer sets and 2 μl cDNA equivalent to 4 ng total RNA input.

Reverse Transcription:

Article Title: Unravelling the synergies: effects of hydrogel mechanics and biofunctionalization on mesenchymal stem cell osteogenic differentiation
Article Snippet: .. RNA samples were extracted and processed with the RNeasy micro kit (Qiagen). cDNA was synthesized from 0.1 mg of total RNA using Maxima Reverse Transcriptase (Fisher Scientific) and primed with oligo-dT primers (Fisher Scientific) and random primers (Fisher Scientific). .. QPCR was performed using a LightCycler 480 Real-Time PCR System (Roche, Meylan, France).

Article Title: Bioreactor-produced iPSCs-derived dopaminergic neuron-containing neural microtissues innervate and normalize rotational bias in a dose-dependent manner in a Parkinson rat model.
Article Snippet: Samples were homogenised in Tri-reagent (Euromedex, ref. TR 118- 200) and RNA was isolated using a standard chloroform/isopropanol protocol [25]. .. RNA was processed and analysed following an adaptation of published methods [26]. cDNA was synthesised from 2 μg of total RNA using Maxima Reverse Transcriptase (Fisher Scientific, ref. 10420100) and primed with oligo-dT primers (Fisher Scientific, ref. 10753741) and random primers (Fisher Scientific 10580091). .. QPCR was performed using a LightCycler® 480 Real-Time PCR System (Roche).

Article Title: Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
Article Snippet: RNA was quantified using a NanoDrop1000 spectrophotometer (Fisher Scientific). .. 400 ng of RNA were reversed transcribed into cDNA using Maxima Reverse Transcriptase (Fisher Scientific). .. Quantitative real-time PCR (qRT-PCR) was performed with PowerUp SYBR Green Master Mix (Applied Biosystems) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

Article Title: Optimized alveolar epithelial cell model for chronic Pseudomonas aeruginosa and Staphylococcus aureus coinfections
Article Snippet: RNA was quantified using a NanoDrop1000 spectrophotometer (Fisher Scientific). .. 400 ng of RNA were reversed transcribed into cDNA using Maxima Reverse Transcriptase (Fisher Scientific). .. Quantitative real-time PCR (qRT-PCR) was performed with PowerUp SYBR Green Master Mix (Applied Biosystems) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

Article Title: Development of an Electrochemical Sensor for SARS-CoV-2 Detection Based on Loop-Mediated Isothermal Amplification.
Article Snippet: For the RT-qPCR, One Step PrimeScript III RT-qPCR Mix (Takara, Japan) was used. .. LAMP mixture buffer for isothermal amplification ThermoPol buffer (10X), MgSO4, dNTPs and Bst DNA polymerase large fragment were purchased from New England Biolabs, UK, and the Evagreen fluorescence dye was purchased from Biotium, EEUU and Maxima Reverse Transcriptase from Fisher Scientific, S.L., betaine, Methylene Blue (MB) and the Tris-EDTA buffer for the collection of the samples were acquired from Sigma Aldrich, UK. .. The RiboGuard RNase inhibitor was purchased from Lucigen, UK.

Article Title: Bioreactor-produced iPSCs-derived dopaminergic neuron-containing neural microtissues innervate and normalize rotational bias in a dose-dependent manner in a Parkinson rat model
Article Snippet: Samples were homogenised in Tri-reagent (Euromedex, ref. TR 118-200) and RNA was isolated using a standard chloroform/isopropanol protocol [ ]. .. RNA was processed and analysed following an adaptation of published methods [ ]. cDNA was synthesised from 2 μg of total RNA using Maxima Reverse Transcriptase (Fisher Scientific, ref. 10420100) and primed with oligo-dT primers (Fisher Scientific, ref. 10753741) and random primers (Fisher Scientific 10580091). .. QPCR was performed using a LightCycler® 480 Real-Time PCR System (Roche).

Article Title: Growth deficiency and enhanced basal immunity in Arabidopsis thaliana mutants of EDM2 , EDM3 and IBM2 are genetically interlinked
Article Snippet: Total RNA of the aerial parts of two weeks old plants was extracted using Trizol reagent (Life technologies) according to the instructions of the manufacturer. .. 1μg of RNA was reversed transcribed into cDNA using Maxima Reverse Transcriptase (Fisher scientific). .. The cDNA products were used for Real-time PCR with CFX CONNECT detection system (Bio-Rad).

Article Title: Repression of apelin Furin cleavage sites provides antimetastatic strategy in colorectal cancer.
Article Snippet: Ribonucleic acid (RNA) extraction, real-time PCR and droplet digital PCR (ddPCR) For real-time PCR, total RNA was extracted using an RNA isolation kit (Macherey-Nagel) including DNase treatment (Qiagen), according to the manufacturer’s instructions, and qPCR data were acquired with the StepOnePlusTM Real-Time PCR System (Applied Biosystems, Courtaboeuf, France), as previously described (Scamuffa et al, 2008). .. For ddPCR, cDNA was synthesized from 2 μg of total RNA using Maxima Reverse Transcriptase (Fisher Scientific) and primed with oligo-dT primers (Fisher Scientific) and random primers (Fisher Scientific). .. PCR were performed in a final volume of 22 μl containing the required QX200 ddPCR EvaGreen Supermix (Bio-Rad) with a final concentration of 150 nM of each primer sets and 2 μl cDNA equivalent to 4 ng total RNA input.

Amplification:

Article Title: Development of an Electrochemical Sensor for SARS-CoV-2 Detection Based on Loop-Mediated Isothermal Amplification.
Article Snippet: For the RT-qPCR, One Step PrimeScript III RT-qPCR Mix (Takara, Japan) was used. .. LAMP mixture buffer for isothermal amplification ThermoPol buffer (10X), MgSO4, dNTPs and Bst DNA polymerase large fragment were purchased from New England Biolabs, UK, and the Evagreen fluorescence dye was purchased from Biotium, EEUU and Maxima Reverse Transcriptase from Fisher Scientific, S.L., betaine, Methylene Blue (MB) and the Tris-EDTA buffer for the collection of the samples were acquired from Sigma Aldrich, UK. .. The RiboGuard RNase inhibitor was purchased from Lucigen, UK.

Fluorescence:

Article Title: Development of an Electrochemical Sensor for SARS-CoV-2 Detection Based on Loop-Mediated Isothermal Amplification.
Article Snippet: For the RT-qPCR, One Step PrimeScript III RT-qPCR Mix (Takara, Japan) was used. .. LAMP mixture buffer for isothermal amplification ThermoPol buffer (10X), MgSO4, dNTPs and Bst DNA polymerase large fragment were purchased from New England Biolabs, UK, and the Evagreen fluorescence dye was purchased from Biotium, EEUU and Maxima Reverse Transcriptase from Fisher Scientific, S.L., betaine, Methylene Blue (MB) and the Tris-EDTA buffer for the collection of the samples were acquired from Sigma Aldrich, UK. .. The RiboGuard RNase inhibitor was purchased from Lucigen, UK.



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